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地高辛的盒子II


相关信息

别名 地高辛高效DNA标记及检测试剂盒
英文名称 DIG-High Prime DNA Labeling and Detection Starter Kit II?
储存条件 20℃保存
单位
规格 50T
罗氏地高辛盒子 11585614910

Principle

The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.
Application
DIG-High Prime is used for the highly efficient random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled probes are generated at high yield within one hour or after overnight incubation. DIG-High Prime labeled DNA probes are used in a variety of hybridization techniques: 
? Southern blots[4]
? Northern blots[3]
? Dot blots[2]
? Colony and plaque hybridizations
? For all types of filter hybridization
? For single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat


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注意:
1. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2. 为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。

货号:11585614910
品牌:Roche
规格:

为科研服务、为生命尽责

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